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. 1977 Sep;4(9):3123-42.
doi: 10.1093/nar/4.9.3123.

The nucleotide sequence surrounding the origin of DNA replication of Col E1

D Bastia
Free PMC article

The nucleotide sequence surrounding the origin of DNA replication of Col E1

D Bastia. Nucleic Acids Res. 1977 Sep.
Free PMC article

Abstract

The DNA of Col E1 replicates from a unique origin located at a distance of 17-19% of the genome length from the single Eco RI clevage site. The nucleotide sequence about this site has been determined by a combination of RNA and DNA sequencing techniques. The principal features of the sequence are two palindromes, one of which resembles a palindrome located in the intercistronic region of 0X174. The sequence also contains stretches of purine and pyrimidine clusters of the following compositions: pAT5G, pC2T5G, pGT5G. The origin sequence demonstrates that initiation of DNA replication takes place in an intercistronic region of Col E1DNA, although the possibility that this region makes small polypeptides 30-40 residues long cannot be strictly eliminated at this time.

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References

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    1. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 - PubMed
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