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. 1977 Sep;4(9):3239-58.
doi: 10.1093/nar/4.9.3239.

Primary structure of baker's yeast tRNAVal 2b

V G Gorbulev, V D Axel'rod, A A Bayev
Free PMC article

Primary structure of baker's yeast tRNAVal 2b

V G Gorbulev et al. Nucleic Acids Res. 1977 Sep.
Free PMC article

Abstract

The minor form of valine tRNA from baker's yeast-tRNAVal 2b--purified by column chromatography was completely digested with guanylo-RNase and pancreatic RNase. The products of these digestions were separated by a combination of thin-layer chromatography on cellulose and high voltage electrophoresis on DEAE-paper and then identified. The halves of tRNA Val 2b were prepared by partial digestion with pancreatic RNase, and their complete guanylo-RNase and pancreatic RNase digests were analysed. Basing on the obtained data the primary structure of baker's yeast tRNA Val 2b was reconstructed.

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