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. 2025 Oct;41(7):e70090.
doi: 10.1002/dmrr.70090.

Proteomic and Metabolomic Interplay in the Regulation of Energy Metabolism During Obesity and Metabolic Syndrome

Carlos Vinicius F da Silva  1 , Carlos José F da Silva  2   3 , Thaís R Cataldi  4 , Carlos A Labate  4 , Youssef B Sade  5 , Sandra Mara N Scapin  5 , Fabiano L Thompson  1 , Cristiane Thompson  1 , Carina Maciel da Silva-Boghossian  6 , Eidy de Oliveira Santos  2   3
Affiliations

Proteomic and Metabolomic Interplay in the Regulation of Energy Metabolism During Obesity and Metabolic Syndrome

Carlos Vinicius F da Silva et al. Diabetes Metab Res Rev. 2025 Oct.

Abstract

Aim: Explore the influence of obesity and Metabolic Syndrome disorders on the plasma proteome and metabolome, through an integrated analysis.

Materials and methods: We investigated metabolic and proteomic alterations associated with obesity and MetS, through mass spectrometry, using plasma samples from 49 volunteers, categorized according to BMI, and MetS.

Results: We identified 231 proteins and 77 metabolites. A subset, including DMBT1, Vanin-1, PTPRJ, β-hydroxybutyrate, α-tocopherol, and 5-oxoproline, emerged as potential key players associated with obesity and MetS. By integrating proteomic and metabolomic data, we were able to construct an interactive network involved in metabolic dysfunction, revealing associations between these molecules and clinical parameters, such as BMI, HOMA-IR and HOMA-β.

Conclusions: Our data suggested an interplay between anti-inflammatory (DMBT1, 3-hydroxybutyrate, 5-oxoproline) and pro-inflammatory pathways (Vanin-1, α-tocopherol, PTPRJ) during disorders of obesity and MetS, demonstrating the potential of an integrated multi-omics approach for a better understanding of the mechanism behind obesity-associated metabolic diseases.

Keywords: metabolic syndrome; metabolome; obesity; omics and interatome; proteome.

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References

    1. World Health Organization, Obesity and Overweight, Accessed on, June 6, 2024, https://www.who.int/news‐room/fact‐sheets/detail/obesity‐and‐overweight.
    1. S. P. Weisberg, D. McCann, M. Desai, M. Rosenbaum, R. L. Leibel, and A. W. Ferrante Jr, “Obesity Is Associated With Macrophage Accumulation in Adipose Tissue,” Journal of Clinical Investigation 112, no. 12 (2003): 1796–1808, https://doi.org/10.1172/jci200319246.
    1. R. Cancello, C. Henegar, N. Viguerie, et al., “Reduction of Macrophage Infiltration and Chemoattractant Gene Expression Changes in White Adipose Tissue of Morbidly Obese Subjects After Surgery‐Induced Weight Loss,” Diabetes 54, no. 8 (2005): 2277–2286, https://doi.org/10.2337/diabetes.54.8.2277.
    1. M. da Saúde, Secretaria de Vigilância em Saúde e Ambiente, Departamento de Análise Epidemiológica e Vigilância de Doenças Não Transmissíveis. Vigitel Brasil 2023: vigilância de fatores de risco e proteção para doenças crônicas por inquérito telefônico: estimativas sobre frequência e distribuição sociodemográfica de fatores de risco e proteção para doenças crônicas nas capitais dos 26 estados brasileiros e no Distrito Federal em 2023 [recurso eletrônico] (Brasília: Ministério da Saúde, 2023).
    1. G. Reaven, “Role of Insulin Resistance in Human Disease,” Diabetes 37, no. 12 (1988): 1595–1607, https://doi.org/10.2337/diab.37.12.1595.

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