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. 1971 Feb;48(2):340-7.
doi: 10.1083/jcb.48.2.340.

The M band. Studies with fluorescent antibody staining

E Kundrat, F A Pepe

The M band. Studies with fluorescent antibody staining

E Kundrat et al. J Cell Biol. 1971 Feb.

Abstract

The M band can be extracted from fibrils suspended in 5 mM Tris buffer, pH 8.0, for 15 min. The M band is completely removed only from fibrils of sarcomere lengths greater than 2.1 micro. Extraction does not alter the fluorescent antimyosin staining pattern of the A band, thus providing strong evidence that no alteration of the structural integrity of the thick filament has occurred. Fluorescent antibody staining of the M band of unextracted fibrils can be prevented specifically by absorbing the fluorescent antibody with extracted M band material prior to staining. This verifies the specificity of the extraction procedure.

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