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. 2025 Sep;53(9):3000605251377660.
doi: 10.1177/03000605251377660. Epub 2025 Sep 29.

Omics-based integrative analysis of sepsis-induced myocardial dysfunction

Jie-Wen Zhao  1 , Zeng-Xiang Dong  2 , Wei Wang  3 , Si-Qi Sheng  3 , Kai-Jiang Yu  1
Affiliations

Omics-based integrative analysis of sepsis-induced myocardial dysfunction

Jie-Wen Zhao et al. J Int Med Res. 2025 Sep.

Abstract

ObjectiveSepsis-induced myocardial dysfunction is a common complication of sepsis, characterized by high mortality and an unclear underlying mechanism. This study conducted an integrative multiomics analysis of mice with sepsis-induced myocardial dysfunction to provide new insights into potential mechanisms.MethodWe constructed an animal model of sepsis-induced myocardial dysfunction and analyzed the metabolomics, transcriptomics, and protein profiles of the heart tissues in the control and experimental groups.ResultsUntargeted metabolomics identified 74 significantly altered metabolites in the positive ion mode, of which 48 were upregulated and 26 downregulated; moreover, 70 significantly altered metabolites were detected in the negative ion mode, with 50 being upregulated and 20 downregulated. Transcriptomics revealed 4831 differentially expressed genes, with 3027 being downregulated and 1804 upregulated. Proteomics identified 107 significant proteins, 94 of which were significantly upregulated and 13 significantly downregulated. Integrated omics analysis revealed three significantly altered metabolites common to both groups: L-glutamate, L-aspartate, and nicotinamide. These metabolites were predominantly involved in nicotinate and nicotinamide metabolism, histidine metabolism, and nitrogen metabolism, potentially related to the pathogenesis of sepsis-induced myocardial dysfunction.ConclusionThe pathogenesis of sepsis-induced myocardial dysfunction in mice may be associated with alterations in nicotinate and nicotinamide metabolism, histidine metabolism, and nitrogen metabolism.

Keywords: Sepsis-induced myocardial dysfunction; metabolic pathways; metabolomics; proteomics; transcriptomics.

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Conflict of interest statement

Declaration of conflicting interestsThe authors declare that they have no competing interests.

Figures

Figure 1.
Figure 1.
Echocardiographic data (a–d) and enzyme-linked immunosorbent assay (ELISA) results show the levels of c-TnI (e). c-TN: cardiac troponin; EF: ejection fraction; FS: fractional shortening; LPS: lipopolysaccharide; LVIDd: left ventricular end-diastolic diameter; LVIDs: left ventricular end-systolic diameter.
Figure 2.
Figure 2.
(a) Principal component analysis diagram and (b) volcano map. FC: fold change; LPS: lipopolysaccharide.
Figure 3.
Figure 3.
(a) Box plot. (b) Principal component analysis diagram. (c) Volcano map and (d) heatmap. FC: fold change; LPS: lipopolysaccharide.
Figure 4.
Figure 4.
(a) Volcano map and (b) heatmap. LPS: lipopolysaccharide.
Figure 5.
Figure 5.
(a) Histograms of differential genes, metabolites, and proteins and (b) the differences in the three amino acids between the control group and experimental group were analyzed. SIMD: sepsis-induced myocardial dysfunction.
Figure 6.
Figure 6.
Omics-based combined analysis of enriched metabolic pathways.
See this image and copyright information in PMC

References

    1. Singer M, Deutschman CS, Seymour CW, et al. The Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3). JAMA 2016; 315: 801–810. - PMC - PubMed
    1. Rudd KE, Johnson SC, Agesa KM, et al. Global, regional, and national sepsis incidence and mortality, 1990–2017: analysis for the Global Burden of Disease Study. Lancet 2020; 395: 200–211. - PMC - PubMed
    1. Martin L, Derwall M, Al Zoubi S, et al. The septic heart: current understanding of molecular mechanisms and clinical implications. Chest 2019; 155: 427–437. - PubMed
    1. Nong Y, Wei X, Yu D. Inflammatory mechanisms and intervention strategies for sepsis-induced myocardial dysfunction. Immun Inflamm Dis 2023; 11: e860. - PMC - PubMed
    1. Dunn WB, Broadhurst D, Begley P; Human Serum Metabolome (HUSERMET) Consortium et al. Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry. Nat Protoc 2011; 6: 1060–1083. - PubMed

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