Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Oct 18;16(21):4591-4.
doi: 10.1021/bi00640a009.

Characterization of the "microprotease" from Bacillus cereus. A zinc neutral endoprotease

B Holmquist

Characterization of the "microprotease" from Bacillus cereus. A zinc neutral endoprotease

B Holmquist. Biochemistry. .

Abstract

The neutral protease isolated from Bacillus cereus (BRL-70) has been purified by affinity chromatography and characterized. The enzyme exhibits a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, has a molecular weight of 34 000 by ultracentrifugation, and contains one enzymatically essential zinc atom per 34 000 g. These data together with the amino acid composition, response to metal substitution, chemical modification, and substrate specificity all indicate that this protease is monomeric and is a typical bacterial neutral metalloprotease.

PubMed Disclaimer

Publication types