EMito-Metrix enables automated evaluation of mitochondrial morphology across species

Eléna Morin^#^{1

2}, Emmanuel Doumard^#^{1

2

3}, Lisa M Hartnell⁴, Beñat Salegi Ansa^{5

6}, Jean-Philippe Leduc-Gaudet⁷, Aurélie Quillien^{1

2}, Jean Nakhle^{1

2}, Séverine Ethuin^{1

2}, Dominique Goudounèche⁸, Bruno Payré⁸, Vanessa Soldan⁹, Stéphanie Balor⁹, Anna Mattout¹⁰, Jacques Rouquette^{1

2}, Laurence Dubois^{1

2}, Coralie Sengenès^{1

2}, Valérie Planat^{1

2}, Louis Casteilla^{1

2}, Armelle Yart^{1

2}, Cédric Dray^{1

2}, Julien Aligon^{1

2

3}, Luigi Ferrucci⁴, Élise Duchesne^{11

12

13

14}, Sabah N A Hussain^{15

16}, Gilles Gouspillou^{16

17

18

19}, Laura Formentini^{5

6}, Arnaud Mourier²⁰, Olivier R Baris²¹, Philippe Valet^{1

2}, Harold Parpex³, Paul Monsarrat^{1

2

22

23}, Mathieu Vigneau^{24

25}, Jean-Philippe Pradère^{26

27}

Affiliations

¹ University of Toulouse, CNRS UMR 5070, INSERM U1301, EFS, ENVT, Institut RESTORE, Toulouse, France.
² IHU HealthAge, Toulouse, France.
³ Université de Toulouse-Capitole, IRIT, (CNRS/UMR 5505), Toulouse, France.
⁴ Translational Gerontology Branch, NIA IRP, NIH, Baltimore, MD, USA.
⁵ Departamento de Biología Molecular, Centro de Biología Molecular 'Severo Ochoa' (CBM, CSIC-UAM), Universidad Autónoma de Madrid (UAM), Madrid, Spain.
⁶ Instituto de Investigación Hospital 12 de Octubre, i+12, Madrid, Spain.
⁷ Research Group in Cellular Signaling, Department of Medical Biology, Université du Québec à Trois-Rivières, Trois-Rivières, Quebec, Canada.
⁸ CMEAB Université Paul Sabatier, Université de Toulouse, Toulouse, France.
⁹ METi, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, Toulouse, France.
¹⁰ MCD, Centre de Biologie Intégrative (CBI), CNRS, Université de Toulouse, UT3, Toulouse, France.
¹¹ School of Rehabilitation Sciences, Faculty of Medicine, Université Laval, Quebec, Quebec, Canada.
¹² Neuromuscular Diseases Interdisciplinary Research Group (GRIMN), Saguenay-Lac-St-Jean Integrated University Health and Social Services Center, Saguenay, Quebec, Canada.
¹³ Center for Interdisciplinary Research in Rehabilitation and Social Integration (Cirris), Capitale-Nationale Integrated University Health and Social Services Center, Quebec, Quebec, Canada.
¹⁴ CHU de Québec - Centre de Recherche de l'Université Laval, Quebec, Quebec, Canada.
¹⁵ Department of Critical Care, McGill University Health Centre, Montreal, Quebec, Canada.
¹⁶ Meakins-Christie Laboratories, McGill University and Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada.
¹⁷ Département des Sciences de l'Activité Physique, Faculté des Sciences, UQAM, Montreal, Quebec, Canada.
¹⁸ Groupe de Recherche en Activité Physique Adaptée, Montreal, Quebec, Canada.
¹⁹ Centre de Recherche de l'Institut Universitaire de Gériatrie de Montréal, Montreal, Quebec, Canada.
²⁰ University of Bordeaux, CNRS, Institut de Biochimie et Génétique Cellulaires (IGBC) UMR, Bordeaux, France.
²¹ University of Angers, MitoLab, Unité MITOVASC, UMR CNRS 6015, INSERM U1083, SFR ICAT, Angers, France.
²² Department of Oral Medicine and Toulouse University Hospital (CHU of Toulouse)-Toulouse Institute of Oral Medicine and Science, Université de Toulouse, Toulouse, France.
²³ Artificial and Natural Intelligence Toulouse Institute ANITI, Université de Toulouse, Toulouse, France.
²⁴ University of Toulouse, CNRS UMR 5070, INSERM U1301, EFS, ENVT, Institut RESTORE, Toulouse, France. mathieu.vigneau@cnrs.fr.
²⁵ IHU HealthAge, Toulouse, France. mathieu.vigneau@cnrs.fr.
²⁶ University of Toulouse, CNRS UMR 5070, INSERM U1301, EFS, ENVT, Institut RESTORE, Toulouse, France. jean-philippe.pradere@inserm.fr.
²⁷ IHU HealthAge, Toulouse, France. jean-philippe.pradere@inserm.fr.

^# Contributed equally.

PMID: 41094017
DOI: 10.1038/s42255-025-01400-z

EMito-Metrix enables automated evaluation of mitochondrial morphology across species

Eléna Morin et al. Nat Metab. 2025.

. 2025 Oct 15.

doi: 10.1038/s42255-025-01400-z. Online ahead of print.

Authors

Affiliations

¹ University of Toulouse, CNRS UMR 5070, INSERM U1301, EFS, ENVT, Institut RESTORE, Toulouse, France.
² IHU HealthAge, Toulouse, France.
³ Université de Toulouse-Capitole, IRIT, (CNRS/UMR 5505), Toulouse, France.
⁴ Translational Gerontology Branch, NIA IRP, NIH, Baltimore, MD, USA.
⁵ Departamento de Biología Molecular, Centro de Biología Molecular 'Severo Ochoa' (CBM, CSIC-UAM), Universidad Autónoma de Madrid (UAM), Madrid, Spain.
⁶ Instituto de Investigación Hospital 12 de Octubre, i+12, Madrid, Spain.
⁷ Research Group in Cellular Signaling, Department of Medical Biology, Université du Québec à Trois-Rivières, Trois-Rivières, Quebec, Canada.
⁸ CMEAB Université Paul Sabatier, Université de Toulouse, Toulouse, France.
⁹ METi, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, Toulouse, France.
¹⁰ MCD, Centre de Biologie Intégrative (CBI), CNRS, Université de Toulouse, UT3, Toulouse, France.
¹¹ School of Rehabilitation Sciences, Faculty of Medicine, Université Laval, Quebec, Quebec, Canada.
¹² Neuromuscular Diseases Interdisciplinary Research Group (GRIMN), Saguenay-Lac-St-Jean Integrated University Health and Social Services Center, Saguenay, Quebec, Canada.
¹³ Center for Interdisciplinary Research in Rehabilitation and Social Integration (Cirris), Capitale-Nationale Integrated University Health and Social Services Center, Quebec, Quebec, Canada.
¹⁴ CHU de Québec - Centre de Recherche de l'Université Laval, Quebec, Quebec, Canada.
¹⁵ Department of Critical Care, McGill University Health Centre, Montreal, Quebec, Canada.
¹⁶ Meakins-Christie Laboratories, McGill University and Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada.
¹⁷ Département des Sciences de l'Activité Physique, Faculté des Sciences, UQAM, Montreal, Quebec, Canada.
¹⁸ Groupe de Recherche en Activité Physique Adaptée, Montreal, Quebec, Canada.
¹⁹ Centre de Recherche de l'Institut Universitaire de Gériatrie de Montréal, Montreal, Quebec, Canada.
²⁰ University of Bordeaux, CNRS, Institut de Biochimie et Génétique Cellulaires (IGBC) UMR, Bordeaux, France.
²¹ University of Angers, MitoLab, Unité MITOVASC, UMR CNRS 6015, INSERM U1083, SFR ICAT, Angers, France.
²² Department of Oral Medicine and Toulouse University Hospital (CHU of Toulouse)-Toulouse Institute of Oral Medicine and Science, Université de Toulouse, Toulouse, France.
²³ Artificial and Natural Intelligence Toulouse Institute ANITI, Université de Toulouse, Toulouse, France.
²⁴ University of Toulouse, CNRS UMR 5070, INSERM U1301, EFS, ENVT, Institut RESTORE, Toulouse, France. mathieu.vigneau@cnrs.fr.
²⁵ IHU HealthAge, Toulouse, France. mathieu.vigneau@cnrs.fr.
²⁶ University of Toulouse, CNRS UMR 5070, INSERM U1301, EFS, ENVT, Institut RESTORE, Toulouse, France. jean-philippe.pradere@inserm.fr.
²⁷ IHU HealthAge, Toulouse, France. jean-philippe.pradere@inserm.fr.

^# Contributed equally.

PMID: 41094017
DOI: 10.1038/s42255-025-01400-z

No abstract available

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Conflict of interest statement

Competing interests: The authors declare no competing interests.

References

1. Lam, J. et al. Cells 10, 2177 (2021). - DOI - PubMed - PMC
1. Kievits, A. J., Lane, R., Carroll, E. C. & Hoogenboom, J. P. J. Microsc. 287, 114–137 (2022). - DOI - PubMed - PMC
1. Gómez-de-Mariscal, E. et al. Nat. Methods 18, 1192–1195 (2021). - DOI - PubMed
1. Conrad, R. & Narayan, K. Cell Syst. 14, 58–71.e5 (2023). - DOI - PubMed - PMC
1. Suga, S. et al. PLoS Biol. 21, e3002246 (2023). - DOI - PubMed - PMC

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EMito-Metrix enables automated evaluation of mitochondrial morphology across species

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EMito-Metrix enables automated evaluation of mitochondrial morphology across species

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Conflict of interest statement

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