Regulation of the immune system by synthetic polynucleotides. V. Effect on cell-associated immunoglobulin receptors and immunological memory
- PMID: 4109807
- PMCID: PMC2139118
- DOI: 10.1084/jem.135.1.45
Regulation of the immune system by synthetic polynucleotides. V. Effect on cell-associated immunoglobulin receptors and immunological memory
Abstract
Addition of polyadenylic-polyuridylic acid in complex form (poly A:U) without antigen to a suspension of spleen cells obtained from BALB/Aj mice primed 6 wk previously with human gamma-globulin (HGG) resulted in an immediate fourfold increase over background number of anti-HGG rosette-forming cells (RFC). Culture of similar cells in the presence of puromycin for 1-6 hr before poly A:U did not significantly reduce the response. Continued culture of primed spleen cells in the presence of poly A:U, resulted in a decrease of RFC to background levels within an hour followed by an increase again 6 hr later. This later increase in RFC was inhibited by addition of puromycin to the culture medium. The nonspecific stimulation by poly A:U of antibody production by primed spleen cells also was induced in vivo. Increases in splenic RFC were detectable 6 hr after intravenous injection of poly A:U alone, without antigen, into primed mice. The response peaked at 18 hr and had dissipated completely within 3 days. A second injection of poly A:U 24 hr or later after the first injection resulted in a second response, similar to the first with respect to kinetics and intensity. Rosette formation by poly A:U-stimulated cells could not be inhibited by mitotic poisons, but was inhibited by treatment of the cells with goat anti-mouse gamma-globulin serum, suggesting that the antibody involved was a 7S gamma-globulin. The decrease in RFC induced by culture of primed cells for 1 hr in poly A:U paralleled a decrease in secondary responsiveness of the cells to antigen. This poly A:U-induced inhibition of secondary responsiveness could be reversed by suspending the treated cells in supernatant fluids derived from poly A:U-stimulated cultures. The reversal was specific in that supernatant fluids removed from bovine serum albumin (BSA)-primed cells by poly A:U did not stimulate the response of HGG-primed cells to HGG. However supernatant fluids from BSA-primed cells caused the production of anti-HGG RFC if BSA rather than HGG was used as triggering antigen. The active factor in the supernatant fluids appeared to be a 7S gamma-globulin since activity was lost after 45 min incubation of the supernatant fluids in the presence of goat anti-mouse 7S gamma-globulin serum.
Similar articles
-
Regulation of the immune system by synthetic polynucleotides. II. Action on peritoneal exudate cells.J Exp Med. 1971 Mar 1;133(3):649-64. doi: 10.1084/jem.133.3.649. J Exp Med. 1971. PMID: 4106807 Free PMC article.
-
Regulation of the immune system by synthetic polynucleotides. 3. Action on antigen-reactive cells of thymic origin.J Exp Med. 1971 Mar 1;133(3):665-76. doi: 10.1084/jem.133.3.665. J Exp Med. 1971. PMID: 4939152 Free PMC article.
-
Polyadenylic: polyuridylic acid-induced determinants of host resistance to cytomegalovirus and their potentiation by hyperthermia.J Immunother (1991). 1992 Aug;12(2):105-14. doi: 10.1097/00002371-199208000-00005. J Immunother (1991). 1992. PMID: 1324000
-
[Selective concurrent suppression of the process of rosette-forming cell accumulation during the immune response].Biull Eksp Biol Med. 1976 Jun;81(6):715-7. Biull Eksp Biol Med. 1976. PMID: 1085182 Russian.
-
Immune responses in vitro. IV. Suppression of primary M, G, and A plaque-forming cell responses in mouse spleen cell cultures by class-specific antibody to mouse immunoglobulins.J Exp Med. 1972 Mar 1;135(3):675-97. doi: 10.1084/jem.135.3.675. J Exp Med. 1972. PMID: 4536706 Free PMC article.
Cited by
-
Studies on membrane-bound receptors for antigen. Preparation of populations of receptor-depleted lymphocytes.J Exp Med. 1973 Oct 1;138(4):875-86. doi: 10.1084/jem.138.4.875. J Exp Med. 1973. PMID: 4355280 Free PMC article.
-
Surface antigens of immunocompetent cells. 3. In vitro studies of the role of B and T cells in immunological memory.J Exp Med. 1972 Oct 1;136(4):663-75. doi: 10.1084/jem.136.4.663. J Exp Med. 1972. PMID: 4560343 Free PMC article.
-
Polyadenylic acid-polyuridylic acid (poly A : U) and experimental murine brucellosis. II. Macrophages as target cells of poly A : U in experimental brucellosis.Immunology. 1978 Jul;35(1):77-84. Immunology. 1978. PMID: 680804 Free PMC article.