Isolation of temperature-sensitive mutants of Schizosaccharomyces pombe
- PMID: 4110142
- PMCID: PMC285166
- DOI: 10.1128/jb.109.2.484-491.1972
Isolation of temperature-sensitive mutants of Schizosaccharomyces pombe
Abstract
Seventy-one mutants of the yeast Schizosaccharomyces pombe that were able to grow on complete medium at 25 C but not at 37 C were isolated. Strains selected for further study showed: (i) single gene mutation and (ii) cell lengthening at the restrictive temperature. Preliminary characterization of 13 mutants is reported. Seven of them have a less pronounced synthesis of deoxyribonucleic acid at the restrictive temperature, and four of them seem to be affected in cell division.
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