Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Aug 29;370(2):121-6.
doi: 10.1007/BF00581684.

Carbonic anhydrase activity of isolated brush border and basal-lateral membranes of renal tubular cells

P J Wistrand, R Kinne

Carbonic anhydrase activity of isolated brush border and basal-lateral membranes of renal tubular cells

P J Wistrand et al. Pflugers Arch. .

Abstract

Carbonic anhydrase activity was found and characterized in isolated homogeneous preparations of microvilli and basal infoldings of the rat renal tubular cells. This membrane-bound activity was about 3% of the total carbonic anhydrase activity of the renal cortex. Compared to the renal cytoplasmic soluble form, the membrane-bound enzymes had the same Km for CO2 and were equally resistant to inhibition by chloride. However, they were less inhibited by different sulfonamides. The implication of the existence of membrane-bound enzymes in the renal tubular cells is discussed.

PubMed Disclaimer

References

    1. Acta Physiol Scand Suppl. 1974;418:1-43 - PubMed
    1. Biochem Pharmacol. 1974 Nov 15;23(22):3153-61 - PubMed
    1. Science. 1967 Jan 6;155(3758):44-7 - PubMed
    1. Adv Exp Med Biol. 1972;28:215-24 - PubMed
    1. Acta Physiol Scand. 1971 Apr;81(4):433-9 - PubMed