Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Sep 21;53(2):149-54.
doi: 10.1007/BF00380459.

Fixation of trypanosomatids for electron microscopy with the glutaraldehyde-tannic acid method

T C Soares, W de Souza

Fixation of trypanosomatids for electron microscopy with the glutaraldehyde-tannic acid method

T C Soares et al. Z Parasitenkd. .

Abstract

Epimastigotes from Trypanosoma cruzi and promastigotes from Herpetomonas samuelpessoai were fixed with glutaraldehyde-tannic acid. Different concentrations of tannic acid were tested. With this technique the cellular membranes appear in negative contrast offering the same aspect as seen in cells fixed in glutaraldehyde only without post-fixation in osmium tetraoxide. An electron-dense deposit appears on the surface which possibly represents positively charged groups. The structure of the sub-pellicular microtubules appears well defined and it was possible to distinguish the 13 protofilaments which compose the microtubule wall.

PubMed Disclaimer

References

    1. Rev Inst Med Trop Sao Paulo. 1964 May-Jun;6:93-100 - PubMed
    1. J Cell Sci. 1969 Jul;5(1):163-93 - PubMed
    1. Parasitology. 1954 May;44(1-2):16-23 - PubMed
    1. Can J Microbiol. 1976 Feb;22(2):197-203 - PubMed
    1. J Cell Biol. 1975 Apr;65(1):227-33 - PubMed