¹H NMR guided isolation of flavans from Broussonetia papyrifera and their α-glucosidase inhibitory activity

Tingting Guo¹, Kepu Huang², Qingfang Wang³, Chen Peng⁴, Xingping Zhang², Congxi Gao², Kou Wang⁵, Hongping He⁶, Mingming Li⁷

Affiliations

¹ School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China; School of Ethnic Medicine and Yunnan Key Laboratory of Dai and Yi Medicines, Yunnan University of Chinese Medicine, Kunming, 650500, China.
² School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, 650500, China.
³ School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China; Baoshan Inspection and Testing Institute, Baoshan, 678000, China.
⁴ School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China.
⁵ School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, 650500, China; Yunnan College of Modern Biomedical Industry, Kunming, 650500, China. Electronic address: wangkou@kmmu.edu.cn.
⁶ School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China. Electronic address: 95431111@qq.com.
⁷ School of Ethnic Medicine and Yunnan Key Laboratory of Dai and Yi Medicines, Yunnan University of Chinese Medicine, Kunming, 650500, China. Electronic address: lmm_xiaobai@126.com.

PMID: 41130345
DOI: 10.1016/j.phytochem.2025.114714

¹H NMR guided isolation of flavans from Broussonetia papyrifera and their α-glucosidase inhibitory activity

Tingting Guo et al. Phytochemistry. 2026 Feb.

. 2026 Feb:242:114714.

doi: 10.1016/j.phytochem.2025.114714. Epub 2025 Oct 21.

Authors

Tingting Guo¹, Kepu Huang², Qingfang Wang³, Chen Peng⁴, Xingping Zhang², Congxi Gao², Kou Wang⁵, Hongping He⁶, Mingming Li⁷

Affiliations

¹ School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China; School of Ethnic Medicine and Yunnan Key Laboratory of Dai and Yi Medicines, Yunnan University of Chinese Medicine, Kunming, 650500, China.
² School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, 650500, China.
³ School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China; Baoshan Inspection and Testing Institute, Baoshan, 678000, China.
⁴ School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China.
⁵ School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, 650500, China; Yunnan College of Modern Biomedical Industry, Kunming, 650500, China. Electronic address: wangkou@kmmu.edu.cn.
⁶ School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China. Electronic address: 95431111@qq.com.
⁷ School of Ethnic Medicine and Yunnan Key Laboratory of Dai and Yi Medicines, Yunnan University of Chinese Medicine, Kunming, 650500, China. Electronic address: lmm_xiaobai@126.com.

PMID: 41130345
DOI: 10.1016/j.phytochem.2025.114714

Abstract

Six undescribed flavans (1-6) and four known analogues (7-10) were isolated from the leaves of Broussonetia papyrifera with the guidance of ¹H NMR. Among them, 1 and 2 were characterized as prenylflavan derivatives with hydroxymethyl and ethoxy moiety, respectively. Their structures were established through comprehensive analysis of spectroscopic data, including HRESIMS, 1D and 2D NMR. The absolute configurations were determined by theoretical calculations of electronic circular dichroism. Notably, compounds 5 and 6 exhibited superior α-glucosidase inhibitory activities with IC₅₀ values of 5.87 and 6.26 μM, respectively. Additionally, molecular docking and molecular dynamics simulations were performed using 5 and 6 with IMA1, an α-glucosidase derived from Saccharomyces cerevisiae, to analyze their potential binding sites.

Keywords: (1)H NMR guided isolation; Broussonetia papyrifera (L.) L'Hér. ex Vent.; Flavans; Moraceae; α-glucosidase.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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¹H NMR guided isolation of flavans from Broussonetia papyrifera and their α-glucosidase inhibitory activity

Affiliations

¹H NMR guided isolation of flavans from Broussonetia papyrifera and their α-glucosidase inhibitory activity

Authors

Affiliations

Abstract

Conflict of interest statement

MeSH terms

Substances

LinkOut - more resources

Full Text Sources