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. 1977 Jun;8(2):133-41.

Partial chemical and immunological characterization of pepsin-solubilized collagen from the tunica albuginea of rat testis

B Denduchis, L Lustig, N N González, R P Puig
  • PMID: 411305

Partial chemical and immunological characterization of pepsin-solubilized collagen from the tunica albuginea of rat testis

B Denduchis et al. Acta Eur Fertil. 1977 Jun.

Abstract

A pepsin-solubilized collagen was isolated from the tunica albuginea of rat testis. Intrinsic viscosity, amino acid and carbohydrate composition were similar to data reported for other soluble collagens. An antiserlm to the pepsin-solubilized collagen was obtained and by indirect immunofluorescence an antigen-antibody reaction was observed in the collagen fibers of the tunica albuginea and in the intertubular collagen fibers of rat testis. By serological and immunofluorescence techniques, cross-reactions with other collagens indicates a lack of organ specificity. A strong cross-reaction between this antiserum and basement membranes from different organs of the rat was also found.

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