Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1972 Jun;23(6):1097-100.
doi: 10.1128/am.23.6.1097-1100.1972.

Agar block technique for identification of mycoplasmas by use of fluorescent antibody

E J Baas, D E Jasper

Agar block technique for identification of mycoplasmas by use of fluorescent antibody

E J Baas et al. Appl Microbiol. 1972 Jun.

Abstract

A procedure for staining mycoplasmata colonies directly on agar blocks for examination by fluorescent microscopy is described. Areas of the agar surface appropriate for staining were demarcated by use of Lucite cylinders. Direct fluorescent-antibody staining was superior to indirect staining. The technique was very useful for determining whether cultures were mixed and for identification of mycoplasmas in either pure or mixed cultures.

PubMed Disclaimer

References

    1. Proc Soc Exp Biol Med. 1967 Jun;125(2):538-43 - PubMed
    1. Acta Pathol Microbiol Scand B Microbiol Immunol. 1970;78(2):149-52 - PubMed
    1. Ann N Y Acad Sci. 1967 Jul 28;143(1):50-8 - PubMed
    1. Proc Soc Exp Biol Med. 1964 Aug-Sep;116:947-50 - PubMed
    1. J Bacteriol. 1968 Nov;96(5):1867-9 - PubMed

LinkOut - more resources