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. 1972 Jul;23(1):7-12.

Evaluation of the immunological specificity of fluorescein-labelled anti-human IgM conjugates

S Chantler, M Haire

Evaluation of the immunological specificity of fluorescein-labelled anti-human IgM conjugates

S Chantler et al. Immunology. 1972 Jul.

Abstract

The immunological specificity of two anti-IgM fluorescein-labelled antisera was evaluated in gel diffusion, by the direct immunofluorescent technique on characterized bone marrow preparations taken from patients with myeloma and by an indirect staining method employing virus-infected cells and selected post-infection sera. The results show that neither gel diffusion nor direct immunofluorescent methods provide a reliable index of specificity for conjugates to be used in indirect procedures.

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References

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    1. J Immunol. 1968 Jun;100(6):1169-75 - PubMed
    1. Immunochemistry. 1969 Jan;6(1):53-66 - PubMed
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