Identification and Analysis of Resistance to Northern Corn Leaf Blight in Maize Germplasm Resources

Abstract

Northern corn leaf blight (NCLB), caused by the fungus Exserohilum turcicum, is one of the most significant foliar diseases in maize worldwide, with its severity being highly influenced by environmental conditions. An effective strategy used to control NCLB involves screening diverse maize germplasm for resistant sources through multi-environment inoculation assays, ultimately aiming to develop resistant varieties. This study systematically evaluated 711 maize germplasm accessions with rich genetic diversity. The evaluation was conducted under four location-year environment combinations (Shangluo, Shaanxi Province, China in 2014-2015 and Xinzhou, Shanxi Province, China in 2021-2022) using artificial inoculation with physiological race 123N (or races 1, 2, 3, N). The results showed that the estimated variances of genotype, environment, and genotype-by-environment interaction were all highly significant (p < 0.01). Significant correlations (p < 0.01) were observed among replicates within each environment, with correlation coefficients (r) ranging from 0.67 to 0.88. At the Xinzhou trial in 2021, four replicates were inoculated with four physiological races (1, 2, 3, and N), revealing highly significant correlations (r = 0.77-0.80, p < 0.01) among them. The disease severity of the tropical germplasm was significantly lower (p < 0.001) than that of the temperate germplasm. Among the temperate subgroups, the PA and PB (groups A and B germplasms derived from modern US hybrids) subgroups exhibited lower disease severity, with the PB subgroup showing the lowest, while the Iodent and Reid subgroups exhibited higher susceptibility. The disease severity responses to the four physiological races were highly positively correlated (r = 0.77-0.80, p < 0.001), and their correlations with the composite inoculation (race 123N) ranged from 0.65 to 0.83. Based on the resistance evaluations across four location-year environment combinations, the 711 maize accessions were classified into five categories: 20 were highly resistant, 236 resistant, 205 moderately resistant, 237 susceptible, and 13 highly susceptible. The findings indicate that the tropical germplasm and the temperate PB subgroup are major sources of NCLB resistance.

Keywords: 123N; Exserohilum turcicum; NCLB; heterotic group; resistance source.

Figure 1

Correlation analysis of northern corn leaf blight severity among replicates for maize inbred lines across four location–year environment combinations. *** denotes p < 0.001.

Figure 2

Disease severity ratings of eight maize heterotic groups across different location–year environment combinations. LRC, Lvda Red Cob; PA and PB, groups A and B germplasms derived from modern US hybrids; SPT, derivatives from the Tangshan Sipingtou Chinese landrace. **** denotes p < 0.001.

Figure 3

Correlation analysis of disease severity following inoculation with four physiological races (1, 2, 3, and N). *** denotes p < 0.001.

Figure 4

Disease severity statistics for eight heterotic groups after separate inoculation with physiological races 1, 2, 3, and N. LRC, Lvda Red Cob; PA and PB, groups A and B germplasms derived from modern US hybrids; SPT, derivatives from the Tangshan Sipingtou Chinese landrace. **** denotes p < 0.001.

Figure 5

Distribution patterns of accessions rated highly resistant (HR) and resistant (R) (A) or susceptible (S) and highly susceptible (HS) (B) across four location–year environment combinations among heterotic groups. LRC, Lvda Red Cob; PA and PB, groups A and B germplasms derived from modern US hybrids; SPT, derivatives from the Tangshan Sipingtou Chinese landrace.