doi: 10.1128/am.24.3.488-494.1972.
Purification of large amounts of murine ribonucleic acid tumor viruses produced in roller bottle cultures
- PMID: 4116893
- PMCID: PMC376544
- DOI: 10.1128/am.24.3.488-494.1972
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Purification of large amounts of murine ribonucleic acid tumor viruses produced in roller bottle cultures
Appl Microbiol.
1972 Sep.
Abstract
Murine ribonucleic acid tumor viruses and C-type virus particles are produced in relatively large quantities in roller bottle cultures. The viruses present in large volumes of culture fluids can be purified by a simple two-step procedure involving polyethylene glycol precipitation and equilibrium centrifugation in sucrose density gradients.
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