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. 1972 Sep;24(3):488-94.
doi: 10.1128/am.24.3.488-494.1972.

Purification of large amounts of murine ribonucleic acid tumor viruses produced in roller bottle cultures

J J Syrewicz, R B Naso, C S Wang, R B Arlinghaus

Purification of large amounts of murine ribonucleic acid tumor viruses produced in roller bottle cultures

J J Syrewicz et al. Appl Microbiol. 1972 Sep.

Abstract

Murine ribonucleic acid tumor viruses and C-type virus particles are produced in relatively large quantities in roller bottle cultures. The viruses present in large volumes of culture fluids can be purified by a simple two-step procedure involving polyethylene glycol precipitation and equilibrium centrifugation in sucrose density gradients.

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References

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