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. 1972 Oct;69(10):2945-9.
doi: 10.1073/pnas.69.10.2945.

Distribution of saccharide residues on membrane fragments from a myeloma-cell homogenate: its implications for membrane biogenesis

H Hirano, B Parkhouse, G L Nicolson, E S Lennox, S J Singer

Distribution of saccharide residues on membrane fragments from a myeloma-cell homogenate: its implications for membrane biogenesis

H Hirano et al. Proc Natl Acad Sci U S A. 1972 Oct.

Abstract

Ferritin conjugates of two plant agglutinins, concanavalin A and ricin, have been used as specific electron microscopic stains for covalently-bound saccharide residues on membrane fragments from a myeloma-cell homogenate. The results indicate that different saccharide residues are uniformly localized to a single surface of each membrane fragment. In particular, the ferritin-concanavalin A conjugate binds exclusively to the cisternal side of membrane fragments of the rough endoplasmic reticulum. If it is postulated that the biogenesis of eukaryotic plasma membranes involves an assembly-line process from precursor intracellular membranes, these observed asymmetric distributions of saccharides on cell membranes can be explained.

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