Investigation of N-desmethyl-acetamiprid-induced neurotransmitter disturbances in cerebrospinal fluid from leukemia patients

Mengya Si¹, Muhammad Nadeem Khan², Hazrat Bilal³, Muhammad Shafiq⁴, Sabir Khan⁵, Xiaoling Guo², Meimei Wang², Jilong Wu², Wang Zhen⁶, Fen Yao⁴, Adela Jing Li⁷, Xiaoyang Jiao⁸

Affiliations

¹ Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Shantou University, Shantou 515063, China.
² Department of Cell Biology and Genetics, Shantou University Medical College, Shantou 515041, China.
³ Jiangxi Key Laboratory of Oncology, Jiangxi Cancer Hospital, The Second Affiliated Hospital of Nanchang Medical College, Jiangxi Cancer Institute, Nanchang, Jiangxi 330029, China.
⁴ Research Institute of Clinical Pharmacy, Department of Pharmacology, Shantou University Medical College, Shantou 515041, China.
⁵ Department of Dermatology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China.
⁶ Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China.
⁷ College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
⁸ Department of Cell Biology and Genetics, Shantou University Medical College, Shantou 515041, China. Electronic address: xyjiao@stu.edu.cn.

PMID: 41173078
DOI: 10.1016/j.etap.2025.104858

Investigation of N-desmethyl-acetamiprid-induced neurotransmitter disturbances in cerebrospinal fluid from leukemia patients

Mengya Si et al. Environ Toxicol Pharmacol. 2025.

. 2025 Oct 30:120:104858.

doi: 10.1016/j.etap.2025.104858. Online ahead of print.

Authors

Mengya Si¹, Muhammad Nadeem Khan², Hazrat Bilal³, Muhammad Shafiq⁴, Sabir Khan⁵, Xiaoling Guo², Meimei Wang², Jilong Wu², Wang Zhen⁶, Fen Yao⁴, Adela Jing Li⁷, Xiaoyang Jiao⁸

Affiliations

¹ Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Shantou University, Shantou 515063, China.
² Department of Cell Biology and Genetics, Shantou University Medical College, Shantou 515041, China.
³ Jiangxi Key Laboratory of Oncology, Jiangxi Cancer Hospital, The Second Affiliated Hospital of Nanchang Medical College, Jiangxi Cancer Institute, Nanchang, Jiangxi 330029, China.
⁴ Research Institute of Clinical Pharmacy, Department of Pharmacology, Shantou University Medical College, Shantou 515041, China.
⁵ Department of Dermatology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China.
⁶ Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China.
⁷ College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
⁸ Department of Cell Biology and Genetics, Shantou University Medical College, Shantou 515041, China. Electronic address: xyjiao@stu.edu.cn.

PMID: 41173078
DOI: 10.1016/j.etap.2025.104858

Abstract

Neonicotinoids (NEOs) and their metabolites impact neuronal function; however, their effects on neurotransmitters remain unexplored. We analyzed cerebrospinal fluid (CSF) from leukemia patients with differing concentrations of N-desmethyl-acetamiprid (N-dm-ACE) for neurotransmitter profiling. Neurotransmitters and biomarkers were measured in CSF, blood, and serum from 39 patients (19 low and 20 high N-dm-ACE) across three age groups: children, adults, and elders. Significant differences (p ≤ 0.05) in neurotransmitters were found among groups, including glutamic acid (253.45 ± 388.16 & 101.11 ± 52.79), aspartic acid (79.64 ± 87.46 & 0.00), tryptophan (325.89 ± 76.47 & 508.09 ± 331.35), and tyrosine (984.41 ± 348.78 ng/mL). Regression analysis indicated that N-dm-ACE is significantly (p < 0.05) positively associated with serum total proteins and negatively with betaine-aldehyde-chloride and ornithine. This suggests that increased accumulation of N-dm-ACE in the CNS may elevate the risk of neurological or neurotoxic conditions. Further studies are needed to better understand the intricate relationship between NEOs, particularly N-dm-ACE, and brain health.

Keywords: Cerebrospinal fluid; Leukemia; Neonicotinoids; Neurological disorders; Neurotoxicity.

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Conflict of interest statement

Declaration of Competing Interest We Authors of this paper declare that we have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Investigation of N-desmethyl-acetamiprid-induced neurotransmitter disturbances in cerebrospinal fluid from leukemia patients

Affiliations

Investigation of N-desmethyl-acetamiprid-induced neurotransmitter disturbances in cerebrospinal fluid from leukemia patients

Authors

Affiliations

Abstract

Conflict of interest statement

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