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. 2025 Dec:239:107316.
doi: 10.1016/j.mimet.2025.107316. Epub 2025 Oct 29.

Streamlined construction of episomal vectors for rapid assessment of fusion phytase display in Saccharomyces cerevisiae

Vo Thi Hoang Lan  1 , Chau Quoc Cuong  1 , Luc Mai Thanh  1 , Nguyen Thi My Trinh  1 , Nguyen Hieu Nghia  2
Affiliations

Streamlined construction of episomal vectors for rapid assessment of fusion phytase display in Saccharomyces cerevisiae

Vo Thi Hoang Lan et al. J Microbiol Methods. 2025 Dec.

Abstract

We developed a fusion phytase combining acid- and alkaline-active phytases, displayed on Saccharomyces cerevisiae cells. Recombinant replicative vectors were constructed to harbor phytase expression cassettes, enabling rapid screening of fusion enzyme constructs. This system provides a preliminary approach for optimizing phytase display prior to genomic integration.

Keywords: Cell surface; Feed enzyme; Phytate; Yeast.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no conflicts of interest.

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