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. 1973 Feb;70(2):526-30.
doi: 10.1073/pnas.70.2.526.

Radioimmunoassay for human alpha 1 -fetoprotein

H K Silver, P Gold, S Feder, S O Freedman, J Shuster
Free PMC article

Radioimmunoassay for human alpha 1 -fetoprotein

H K Silver et al. Proc Natl Acad Sci U S A. 1973 Feb.
Free PMC article

Abstract

A method of radioimmunoassay has been developed for the quantitation of alpha(1)-fetoprotein in human serum. The assay requires 20 mul of serum, can be completed in 8 hr, and can reproducibly detect concentrations of 20 ng of alpha(1)-fetoprotein per ml of serum. Hence, the method is about 500-fold more sensitive for the detection of alpha(1)-fetoprotein than the Ouchterlony teohnique currently in general use. The procedure is of potential clinical value as an aid in the diagnosis of cancer and a number of noncancerous hepatic diseases.

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