Identification of proteins with variable levels of post-translational modifications in human temporal lobe epilepsy

Abstract

Comparative mass spectrometry analysis of hippocampal tissue samples from patients with sclerotic and non-sclerotic temporal lobe epilepsy and nonepileptic patients was undertaken to identify differences in the levels of protein post-translational modifications (PTMs). The original proteomic data obtained by Mathoux et al. [DOI: 10.1172/jci.insight.188612] and deposited in the PRIDE repository (PXD064519) were used in this work. Our reanalysis of the comparative proteomic data identified 53 proteins with PTMs (phosphorylation, methylation, acetylation, and citrullination) that exhibited significant changes in the levels of individual modified peptides. According to the published original data, all 53 proteins are involved in processes associated with neurological diseases in general and epileptogenesis in particular. The analysis identified PTMs of proteins that could play an important role in the pathogenesis of neurological diseases.

Keywords: bioinformatics; epilepsy; post-translational modifications.