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Review
. 2025 Sep 25:35:102358.
doi: 10.1016/j.mtbio.2025.102358. eCollection 2025 Dec.

Chitosan nanoparticles: Green synthesis, biological activities, and sustainable frontiers in targeted drug delivery and cancer nanomedicine - A comprehensive review

Mohamed T El-Saadony  1 , Ahmed M Saad  2 , Mahmoud Sitohy  2 , Samar Sami Alkafaas  3 , Mthokozisi Dladla  4 , Soumya Ghosh  5 , Dina Mostafa Mohammed  6 , Tarek N Soliman  7 , Essam H Ibrahim  8 , Mohamed A Fahmy  1 , Juwan S AbuQamar  9 , Khaled A El-Tarabily  10
Affiliations
Review

Chitosan nanoparticles: Green synthesis, biological activities, and sustainable frontiers in targeted drug delivery and cancer nanomedicine - A comprehensive review

Mohamed T El-Saadony et al. Mater Today Bio. .

Abstract

Chitosan, a biopolymer derived from chitin-the second most abundant natural polysaccharide found in crustaceans such as crabs, lobsters, and shrimp-has become a cornerstone in biomedical innovation. Its unique properties, including hydrophilicity, biocompatibility, biodegradability, low toxicity, and intrinsic cationic nature, make it an ideal candidate for the development of sustainable and multifunctional nanomaterials. Chitosan nanoparticles (CNPs), distinguished by their nanoscale size and enhanced physicochemical characteristics, offer significant advantages in biomedicine, particularly in diagnostic imaging as targeted delivery systems for drugs, genes, and biomolecules in cancer therapy. The green synthesis of CNPs through methods such as microemulsion, polyelectrolyte complexation, ionic gelation, emulsification-solvent diffusion, and reverse micellization further emphasizes their eco-friendly and sustainable production. Recognized as a Generally Recognized as Safe (GRAS) material by the USA Food and Drug Administration, chitosan is widely accepted for use in biomedical applications. This review comprehensively explores the structural features, environmentally friendly synthesis strategies, and advanced characterization techniques of CNPs. Moreover, it highlights their interdisciplinary biomedical applications, including drug delivery via ocular, oral, pulmonary, vaginal, and nasal routes, as well as their promising role in tissue engineering and cancer therapy. By integrating green chemistry principles with advanced biomedical design, CNPs are reshaping the future of nanomedicine, offering sustainable and targeted therapeutic solutions.

Keywords: Biomedical applications; Cancer therapy; Green synthesis; Tissue engineering.

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Conflict of interest statement

Authors declare no conflict of interests.

Figures

Image 1
Graphical abstract
Fig. 1
Fig. 1
The average particle size (nm) of chitosan nanoparticles formed from various synthesis techniques.
Fig. 2
Fig. 2
Diverse applications of chitosan nanoparticles (CNPs) in vitro and in vivo.
Fig. 3
Fig. 3
Tree map illustrating the diverse applications of chitosan nanoparticles according to their commercial availability, with higher percentages indicating greater applicability and availability, and lower percentages signifying reduced applicability and restricted availability.
Fig. 4
Fig. 4
The antibacterial mechanism of chitosan nanoparticles (CNPs) in combating bacterial infections.
Fig. 5
Fig. 5
The antiviral mechanism of chitosan nanoparticles (CNPs) in the inhibition of viral infections.
Fig. 6
Fig. 6
The anti-carcinogenic mechanism of chitosan nanoparticles (CNPs), including detailed signaling pathways. ROS, reactive oxygen species; ER, endoplasmic reticulum.
Fig. 7
Fig. 7
The anti-diabetic mechanism of chitosan nanoparticles (CNPs). IRS-1: insulin receptor substrate 1, PI3K: phosphoinositide 3-Kinase, PDK: phosphoinositide-dependent kinase, AKT: protein kinase B, and GSK-3β: glycogen synthase kinase-3 Beta.
Fig. 8
Fig. 8
The healing mechanism of skin repair using chitosan nanoparticles (CNPs).
See this image and copyright information in PMC

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