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. 1973 Mar;7(3):487-92.
doi: 10.1128/iai.7.3.487-492.1973.

Immunosorbent for the isolation of specific antibodies against mannan: localization of antigens in yeast cell walls

H Gerber, M Horisberger, H Bauer

Immunosorbent for the isolation of specific antibodies against mannan: localization of antigens in yeast cell walls

H Gerber et al. Infect Immun. 1973 Mar.

Abstract

A procedure is given for the insolubilization of yeast mannan by entrapment in a polyacrylamide gel. Specific anti-mannan antibodies were separated by immunosorption from all other antibodies of a rabbit antiserum against whole, Formalininactivated Candida utilis cells. Colloidal gold coated with the anti-mannan and anti-non-mannan antibody fractions was used for the ultrastructural visualization of cell wall antigens. A control was performed using normal rabbit serum. Inhibition of anti-mannan antibodies was obtained with mannan oligosaccharides, and none of the antibody fractions showed marking of Saccharomyces cerevisiae cell walls.

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References

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