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Published Erratum
. 2025 Nov 25;122(47):e2529649122.
doi: 10.1073/pnas.2529649122. Epub 2025 Nov 19.

Correction for Hao et al., Synthetical lethality of Werner helicase and mismatch repair deficiency is mediated by p53 and PUMA in colon cancer

No authors listed
Published Erratum

Correction for Hao et al., Synthetical lethality of Werner helicase and mismatch repair deficiency is mediated by p53 and PUMA in colon cancer

No authors listed. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Fig. 5.
Fig. 5.
Inducible knockdown of WRN suppresses xenograft growth in a PUMA-dependent fashion. Parental (WT), CH3+5, and PUMA-KO HCT116 cells with Dox inducible expression of WRN short hairpin RNA (Sh WRN) were established as described in Materials and Methods. (A) Western blotting of indicated proteins in two independent clones of WT, CH3+5, and PUMA-KO Sh WRN HCT116 cells with induction of Sh WRN by Dox (0.2 μg/mL) treatment for indicated time. (B) MTS analysis of viability of indicated cells with induction of Sh WRN as in (A) at indicated time points. Results were expressed as mean ± SD of three independent experiments. (C and D) Nude mice were implanted with 2 × 106 of WT (clone 1), CH3+5, or PUMA-KO Sh WRN HCT116 cells to establish xenograft tumors. Tumor bearing mice were randomized and treated with 18% Protein Rodent Diet ±Dox hyclate (625 mg/kg). (C) Tumor volume at indicated time points after treatment was measured and plotted with P values for indicated comparisons (N = 6 in each group). (D) Tumor sections from mice treated as in (C) for 13 d were analyzed by immunostaining for active caspase 3 expression. (Left) Representative staining pictures with arrows indicating examples of positive signals (Scale bars: 25 μm); (Right) Quantification of positive signals showing means ± SEM of each field in three fields per mouse (N = 3 in each group). *P < 0.05; **P < 0.01; ***P < 0.001.
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