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Published Erratum
. 2025 Nov 20;146(21):2613.
doi: 10.1182/blood.2025031937.

Neri P, Ren L, Azab AK, et al. Integrin β7-mediated regulation of multiple myeloma cell adhesion, migration, and invasion. Blood. 2011;117(23):6202-6213

No authors listed
Published Erratum

Neri P, Ren L, Azab AK, et al. Integrin β7-mediated regulation of multiple myeloma cell adhesion, migration, and invasion. Blood. 2011;117(23):6202-6213

No authors listed. Blood. .
No abstract available

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Figures

Figure 2.
Figure 2.
Effects of ITGB7 silencing on adhesion, migration, and invasion of MM cells. (A) Adhesion of calcein-AM–labeled ITGB7silenced (ITGB7 shRNA2 and ITGB7 shRNA3) versus ITGB7positive (scrambled shRNA) and parental (nontransfected) MM1S and H929 cells to BMSCs, FN, and E-CDH–coated 96-well microplates. Unattached cells were washed and adherent cells were measured in a fluorescence plate reader. Data are presented as percentage of respective controls (mean ± SD of triplicates from 3 independent experiments). (B) Transwell migration (8-μm pores; Costar) of calcein-AM–labeled ITGB7silenced (ITGB7 shRNA2 and ITGB7 shRNA3) vs ITGB7positive (scrambled shRNA) and parental (nontransfected) MM1S and H929 cells to RPMI serum-free media (cnt) or RPMI supplemented with SDF-1α (10 and 20nM). The fluorescence values, quantitated in a fluorescence multiwell plate reader using the 494/517nM filter set; percentage of migrating cells to SDF-1α versus control (serum-free RPMI) are shown. Data are presented as the mean ± SD of triplicates from 3 independent experiments. (C-D) Shown is a representative transwell Matrigel invasion of ITGB7silenced (ITGB7 shRNA2 and ITGB7 shRNA3) vs ITGB7positive (scrambled shRNA) and parental (nontransfected) MM1S and H929 cells under the conditions described in “Transwell migration assay and invasion studies.” Cells that invaded the Matrigel-coated filters are stained with crystal violet and counted using an inverted microscope. Images were acquired with a bright light Olympus BX5 microscope and multispectral camera (Nuance FX; CRi). 40× magnification.
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