Effect of alkaline electrolyzed water on lipid, protein changes, and microbial diversity of frozen-stored Dosidicus gigas based on multi-omics analysis

Abstract

This study evaluates the feasibility of replacing compound phosphate (CPH) with alkaline electrolyzed water (AEW) for the frozen storage of Dosidicus gigas over 120 days. Using water retention performance analysis, multi-omics, and microbial diversity assessment methods, this study investigates the effects of AEW treatment during the frozen storage of Dosidicus gigas. The results revealed that AEW significantly improved the water retention performance of frozen-stored Dosidicus gigas, maintained higher muscle springiness, and effectively reduced lipid oxidative degradation compared to the pure water treatment. Additionally, compared to the CPH, AEW stabilized the cellular skeleton and increased heat shock protein content, reducing water loss. AEW reduced the abundance of spoilage bacteria such as Psychrobacter and Cetobacterium, with antibacterial effects superior to those of CPH, delaying Dosidicus gigas spoilage. In summary, AEW can serve as a green alternative to CPH for frozen storage of Dosidicus gigas.

Keywords: Alkaline electrolyzed water; Dosidicus gigas; Frozen storage; Multi-omics; Water retention performance.