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. 1973;14(3):474-88.
doi: 10.1186/BF03547434.

Serological cross-reactions between different brucella species and Yersinia enterocolitica. Agglutinating activity of 19 S and 7 S antibodies aginst somatic antigen of Brucella abortus and Yersinia enterocolitica type IX

B Hurvell

Serological cross-reactions between different brucella species and Yersinia enterocolitica. Agglutinating activity of 19 S and 7 S antibodies aginst somatic antigen of Brucella abortus and Yersinia enterocolitica type IX

B Hurvell. Acta Vet Scand. 1973.

Abstract
in English, Swedish

Antisera from rabbits immunized with acetone-killed whole cells and with lipopolysaccharides from Brucella abortus (B.a.) and Yersinia enterocolitica type IX (Y.e.) were gel-filtered on Sephadex G-200 columns.

All the antisera had serologically cross-reacting agglutinins against B.a. and Y.e. both in the 19 S and in the 7 S antibody fractions.

The homologous and the heterologous agglutinating activity of 19 S and of 7 S antibodies was tested before and after treatment with dithiothreitol (DTT) and subsequent alkylation. Unlike 19 S antibodies, 7 S antibodies were resistant to the DTT reduction.

The results from heterologous absorptions of the respective 19 S and 7 S antibody fractions indicated that 19 S antibodies both to B.a. and to Y.e. had a greater tendency towards being absorbed to the cross-reacting antigenic determinants than had the corresponding 7 S antibodies. The agglutination titres for 19 as well as 7 S antibody fractions derived from the immunizations with B.a. (both whole cells and LPS) fell more markedly after heterologous absorptions than did the analogous titres for corresponding Y.e. antibody fractions. Possible explanations of these differences are discussed.

Antisera från kaniner immuniserade med dels acetonavdödade helceller och dels med lipopolysackarider från Brucella abortus (B.a.) och Yersinia enterocolitica typ IX (Y.e.) gelfiltrerades på Sephadex G-200 kolonner.

Samtliga antisera hade serologiskt korsreagerande agglutininer root B.a. och Y.e. i både 19 S och 7 S antikroppsfraktionerna.

Den homologa och heterologa agglutinationsaktiviteten hos respektive 19 S och 7 S antikroppar testades före och efter behandling med dithiothreitol (DTT) och efterföljande alkylering. 7 S antikropparna var i motsats till 19 S antikropparna resistenta mot DTT-reduceringen.

Resultaten från gjorda heterologa absorptioner av respektive 19 S och 7 S antikroppsfraktioner tyder på att 19 S antikroppar mot såväl B.a. som Y.e. hade större benägenhet att absorberas till de korsreagerande antigena determinanterna än vad motsvarande 7 S antikroppar hade. Agglutinationstitrarna för såväl 19 S som 7 S antikroppsfraktioner härrörande från immuniseringarna med B.a. (både helceller och LPS) sjönk kraftigare efter heterologa absorptioner än vad de analoga titrarna för motsvarande Y.e.-antikroppsfraktioner gjorde efter heterologa absorptioner. Möjliga förklaringar till dessa differenser diskutterades.

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References

    1. Ahvonen P, Jansson E, Aho K. Marked cross-agglutination between Brucellae and a subtype of Yersinia enterocolitica. Acta path. microbiol. scand. 1969;75:291–295. - PubMed
    1. Akkermans J P W M, Hill W K W. Yersinia enterocolitica serotype 9-infectie als störend element bij de serologische diagnostiek van brucella-infecties bij het varken. (Yersinia enterocolitica serotype 9-infections as a disturbing factor in serological demonstration of brucella-infection in swine) T. Diergeneesk. 1971;24:1654–1662.
    1. Corbel M J, Cullen G A. Differentiation of the serological response to Yersinia enterocolitica and Brucella abortus in cattle. J. Hyg. (Lond.) 1970;68:519–530. - PMC - PubMed
    1. Daguillard F, Edsall G. The agglutinating and bactericidal activity of IgM and IgG antibodies to the 9 and 12 factors of Salmonella typhi 0 901. J. Immunol. 1968;100:1112–1120. - PubMed
    1. Deutsch H F, Morton J I. Dissociation of human serum macroglobulin. Science. 1957;13:600–601. doi: 10.1126/science.125.3248.600. - DOI - PubMed

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