Position of the mutation in beta-galactosidase ochre mutant U118
- PMID: 412841
- PMCID: PMC222035
- DOI: 10.1128/jb.133.1.437-438.1978
Position of the mutation in beta-galactosidase ochre mutant U118
Abstract
The Escherichia coli lacZ ochre mutant strain U118 was converted to an amber mutant and suppressed with supF, which inserts tyrosine. Enzymatically active beta-galactosidase was isolated. It contained tyrosine at residue number 17 instead of glutamic acid as in wild type.
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