Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1974 Mar;13(3):577-83.
doi: 10.1128/JVI.13.3.577-583.1974.

Polymerase activity of Pichinde virus

M F Carter, N Biswal, W E Rawls

Polymerase activity of Pichinde virus

M F Carter et al. J Virol. 1974 Mar.

Abstract

Pichinde virus, a member of the arenavirus group, was examined for polymerase activity. Purified virus was found to contain RNA-dependent RNA polymerase but not RNA-dependent DNA polymerase activity. Since RNase but neither DNase nor actinomycin D inhibited the endogenous polymerase reaction, RNA of the virus appeared to be used as the template. The divalent cations Mg(2+) and Mn(2+) were required for optimal reactivity. The RNA product was partially resistant to RNase and the resistant portion had a sedimentation coefficient of 22 to 26S in sucrose gradients.

PubMed Disclaimer

References

    1. Anal Biochem. 1968 Feb;22(2):195-210 - PubMed
    1. J Virol. 1968 Nov;2(11):1346-52 - PubMed
    1. Biochem Biophys Res Commun. 1968 Dec 30;33(6):895-901 - PubMed
    1. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1462-9 - PubMed
    1. Biochim Biophys Acta. 1969 Jan 21;174(1):1-11 - PubMed

MeSH terms

LinkOut - more resources