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. 1974 Feb;27(2):162-7.
doi: 10.1136/jcp.27.2.162.

Evans blue as counterstain in the demonstration of muscle antibodies by immunofluorescence in myasthenia gravis

O Closs, J A Aarli

Evans blue as counterstain in the demonstration of muscle antibodies by immunofluorescence in myasthenia gravis

O Closs et al. J Clin Pathol. 1974 Feb.

Abstract

The binding of muscle antibodies to skeletal muscle was studied by immunofluorescence techniques. The non-specific fluorescence was not significantly reduced by absorption of the conjugate by tissue powder but was abolished by counterstaining with a 0.1% Evans blue. Possible explanations for this effect are discussed. By this method, immunofluorescence may become more useful in the demonstration of muscle antibodies.

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