This is a preprint.
Orchestrating Spatial Transcriptomics Analysis with Bioconductor
- PMID: 41332620
- PMCID: PMC12667768
- DOI: 10.1101/2025.11.20.688607
Orchestrating Spatial Transcriptomics Analysis with Bioconductor
Abstract
Spatial transcriptomics technologies provide spatially-resolved measurements of gene expression through assays that can either target selected genes or capture transcriptome-wide expression profiles. The complexity and variability of these technologies and their associated data necessitate multi-step workflows integrating diverse computational methods and software packages. We provide a freely accessible, open-source, continuously updated and tested online book containing reproducible code examples, datasets, and discussion about data analysis workflows for spatial omics data using Bioconductor in R, including interoperability with Python.
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