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. 1974 Jun 21;184(4143):1297-8.
doi: 10.1126/science.184.4143.1297.

Cell-cycle analysis in 20 minutes

H A Crissman, R A Tobey

Cell-cycle analysis in 20 minutes

H A Crissman et al. Science. .

Abstract

Mithramycin added to mammalian cells fixed in aqueous ethanol is bound to DNA and fluoresces in direct proportion to the cellular DNA content. Quantitative fluorescence measurement by means of a high-speed flow system instrument provides a rapid method for cell-cycle analysis and for the first time permits continuous monitoring of cell-cycle kinetics during ongoing experiments.

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