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. 2026 Jan:240:107351.
doi: 10.1016/j.mimet.2025.107351. Epub 2025 Dec 2.

A qPCR assay for the detection and quantification of Pseudomonas syringae pv. syringae in susceptible tissues of sweet cherry

Yerko Lovera  1 , Juan San Martin  1 , Braulio Ruiz  1 , Richard M Bastías  1 , Macarena Gerding  1 , Juan Hirzel  2 , Ernesto Moya-Elizondo  3
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A qPCR assay for the detection and quantification of Pseudomonas syringae pv. syringae in susceptible tissues of sweet cherry

Yerko Lovera et al. J Microbiol Methods. 2026 Jan.

Abstract

This study validated a qPCR assay for quantifying Pseudomonas syringae pv. syringae across three growth stages, using syrB gene as target and PVPP modification in extraction protocol, demonstrating high sensitivity and accuracy compared to the traditional drop plate method, which often overestimates bacterial loads. Results support improved disease management strategies.

Keywords: Absolute qPCR; Bacterial canker; Prunus avium.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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