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. 1974 Jun;14(6):439-53.
doi: 10.1016/S0006-3495(74)85925-4.

Differential light scattering from spherical mammalian cells

A Brunsting, P F Mullaney

Differential light scattering from spherical mammalian cells

A Brunsting et al. Biophys J. 1974 Jun.

Abstract

The differential scattered light intensity patterns of spherical mammalian cells were measured with a new photometer which uses high-speed film as the light detector. The scattering objects, interphase and mitotic Chinese hamster ovary cells and HeLa cells, were modeled as (a) a coated sphere, accounting for nucleus and cytoplasm, and (b) a homogeneous sphere when no cellular nucleus was present. The refractive indices and size distribution of the cells were measured for an accurate comparison of the theoretical model with the light-scattering measurements. The light scattered beyond the forward direction is found to contain information about internal cellular morphology, provided the size distribution of the cells is not too broad.

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