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. 1974 Jul;10(1):42-7.
doi: 10.1128/iai.10.1.42-47.1974.

Isolation of skin test-active preparations from yeast-phase cells of Blastomyces dermatitidis

R A Cox, H W Larsh

Isolation of skin test-active preparations from yeast-phase cells of Blastomyces dermatitidis

R A Cox et al. Infect Immun. 1974 Jul.

Abstract

Cell wall and cytoplasmic fractions were isolated from mechanically disrupted yeast-phase cells of Blastomyces dermatitidis in an effort to obtain a reliable skin-test antigen. The biological activities of these fractions were compared with those of two blastomycin preparations. The cytoplasmic antigens exhibited a low index of specificity yet exceeded the specificities of the blastomycins. The skin test-active component(s) of the cytoplasmic material had a molecular weight between 10,000 and 30,000 and could easily be concentrated by ultrafiltration on a PM-10 membrane. Unlike the cytoplasmic antigens and blastomycins, an alkali-soluble, water-soluble cell wall antigen effectively distinguished guinea pigs that were sensitized to B. dermatitidis from those sensitized to Histoplasma capsulatum. The biological activity of the yeast-phase antigen could be quantitated, on a weight basis, after purification by ultrafiltration (PM-10 membrane).

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References

    1. Infect Immun. 1972 Apr;5(4):449-53 - PubMed
    1. Am Rev Tuberc. 1958 Jun;77(6):983-9 - PubMed
    1. J Invest Dermatol. 1955 Apr;24(4):447-54 - PubMed

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