doi: 10.1016/j.cpsurg.2025.101920.
Epub 2025 Oct 17.
Transforming orthotopic liver transplantation: Innovative dry-lab simulation model in mice
Anas Amin Preukschas
1
, Rainer Grotelüschen
1
, Ryosuke Nakano
2
, Mitsufuji Suguru
3
, Shinichiro Yokota
4
, Philipp Seeger
1
, Hans Christian Schmidt
5
, Jöran Lücke
6
, Dimitra E Zazara
7
, Morsal Sabihi
6
, Eleftherios D Papazoglou
6
, Alessandro Parente
8
, Charles-Henri Wassmer
9
, Nicola Colucci
10
, Panagis M Lykoudis
11
, Ioannis A Ziogas
12
, Dimitrios Moris
13
, Baris Mercanoglu
1
, Stephan Bennemann
14
, Abdulrahman Al-Harazi
15
, Michael F Nentwich
16
, Felix Nickel
1
, Oliver Mann
1
, Jakob R Izbicki
1
, Thilo Hackert
1
, Jun Oh
17
, Maja Segedi
18
, Michael Bleszynski
18
, Stephanie Chartier-Plante
18
, David Harriman
19
, Kim C Wagner
20
, Karl J Oldhafer
20
, Ansgar W Lohse
21
, Petra Arck
22
, Gabriel E Gondolesi
23
, Samuel Huber
24
, Blayne A Sayed
25
, Morgan Vandermeulen
26
, Graziano Oldani
27
, Sasha Belenkov
28
, Jun Li
1
, Christian Tomuschat
29
, Tobias Dust
30
, Ane Miren Andres Moreno
31
, Emmanouil Giorgakis
32
, Megan A Adams
33
, George Mazariegos
34
, Tao Zhang
35
, Angus W Thomson
36
, Anastasios D Giannou
37
Affiliations
Affiliations
- 1 Department of General, Visceral and Thoracic Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 2 Department of Surgery, Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA; Department of Gastroenterological and Transplant Surgery, Graduate School of Biomedical and Health Sciences Hiroshima University, Hiroshima, Japan.
- 3 Department of Surgery, Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA.
- 4 Division of Abdominal Transplant, Department of Surgery, Stanford University School of Medicine, Stanford, CA.
- 5 Research Laboratory W23, Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; SimLab - Students for Surgery, Student research group, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 6 Department of General, Visceral and Thoracic Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Section of Molecular Immunology and Gastroenterology, I. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 7 Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, Hamburg, Germany; University Children's Hospital, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Division for Experimental Feto-Maternal Medicine, Department of Obstetrics and Fetal Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 8 Department of Hepatopancreatobiliary and Liver Transplant Surgery, Queen Elizabeth Hospital, Birmingham, UK; Institute of Liver Studies, King's College Hospital NHS Foundation Trust, London, UK.
- 9 Hepatology and Transplantation Laboratory, Department of Surgery, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
- 10 Department of Surgery, University of Cambridge, Cambridge, UK; Division of General Surgery, Department of Surgery, University of British Columbia, Vancouver, British Columbia, Canada; Hepatopancreatobiliary and Liver Transplant Surgery, Vancouver General Hospital, Vancouver, British Columbia, Canada.
- 11 4th Department of Surgery, National and Kapodistrian University of Athens, Athens, Greece; Division of Surgery & Interventional Science, University College London (UCL), UK.
- 12 Division of Transplant Surgery, Department of Surgery, University of Colorado Anschutz Medical Campus and Children's Hospital Colorado, Aurora, CO.
- 13 MedStar Georgetown Transplant Institute, MedStar Georgetown University Hospital, Washington, DC.
- 14 Semmelweis University, Faculty of Medicine, Department of Surgery, Transplantation, and Gastroenterology, Budapest, Hungary.
- 15 SimLab - Students for Surgery, Student research group, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 16 Department of Intensive Care Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 17 University Children's Hospital, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 18 Division of General Surgery, Department of Surgery, University of British Columbia, Vancouver, British Columbia, Canada; Hepatopancreatobiliary and Liver Transplant Surgery, Vancouver General Hospital, Vancouver, British Columbia, Canada.
- 19 Department of Urologic Sciences, University of British Columbia, Vancouver, Canada.
- 20 Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, Asklepios Hospital Barmbek, Hamburg, Germany; Semmelweis University Budapest, Asklepios Campus Hamburg, Hamburg, Germany.
- 21 Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 22 Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Division for Experimental Feto-Maternal Medicine, Department of Obstetrics and Fetal Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 23 Division of Transplant Surgery, Department of Surgery, University of Colorado Anschutz Medical Campus and Children's Hospital Colorado, Aurora, CO; General Surgery, Liver, Pancreas and Intestinal Transplantation, Hospital Universitario, Fundacion Favaloro, Buenos Aires, Argentina.
- 24 Section of Molecular Immunology and Gastroenterology, I. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 25 Department of Surgery, University of Toronto, Toronto, ON, Canada; HBP and Multi Organ Transplant Program, Division of General Surgery, University Health Network, HPB Surgical Oncology, Toronto, ON, Canada.
- 26 Department of Visceral Surgery and Transplantation, CHU Liege, Liege, Belgium; Centre de Recherche et d'Enseignement du Département de Chirurgie (CREDEC), GIGA Metabolism, University of Liege, Liege, Belgium.
- 27 Hepatology and Transplantation Laboratory, Department of Surgery, Faculty of Medicine, University of Geneva, Geneva, Switzerland; Division of General Surgery, Department of Surgery, University of British Columbia, Vancouver, British Columbia, Canada; Hepatopancreatobiliary and Liver Transplant Surgery, Vancouver General Hospital, Vancouver, British Columbia, Canada.
- 28 PerkinElmer. Inc, Waltham, MA.
- 29 Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 30 Department of Trauma and Orthopedic Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 31 Transplant Group, La Paz University Hospital Health Research Institute (IdiPAZ), Madrid, Spain; Department of Pediatric Surgery, La Paz University Hospital, Madrid, Spain.
- 32 Division of Abdominal Transplantation, Department of Surgery, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC; Division of Solid Organ Transplant, Department of Surgery, University of Arkansas for Medical Sciences, Little Rock, AR.
- 33 Division of Surgery & Interventional Science, University College London (UCL), UK.
- 34 Department of Transplant Surgery, UPMC Children's Hospital, Pittsburgh, PA; Hillman Center for Pediatric Transplantation, Thomas E. Starzl Transplantation Institute, UPMC Children's Hospital of Pittsburgh, Pittsburgh, PA.
- 35 Department of Gastric Surgery, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, PR China.
- 36 Department of Surgery, Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA; Department of Immunology, University of Pittsburgh, Pittsburgh, PA.
- 37 Department of General, Visceral and Thoracic Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Section of Molecular Immunology and Gastroenterology, I. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Division of General Surgery, Department of Surgery, University of British Columbia, Vancouver, British Columbia, Canada; Hepatopancreatobiliary and Liver Transplant Surgery, Vancouver General Hospital, Vancouver, British Columbia, Canada; Semmelweis University, Faculty of Medicine, Department of Surgery, Transplantation, and Gastroenterology, Budapest, Hungary; Department of Intensive Care Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Semmelweis University Budapest, Asklepios Campus Hamburg, Hamburg, Germany; Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. Electronic address: a.giannou@uke.de.
- PMID: 41354508
- DOI: 10.1016/j.cpsurg.2025.101920
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Transforming orthotopic liver transplantation: Innovative dry-lab simulation model in mice
Anas Amin Preukschas et al.
Curr Probl Surg.
2025 Dec.
Free article
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doi: 10.1016/j.cpsurg.2025.101920.
Epub 2025 Oct 17.
Authors
Anas Amin Preukschas
1
, Rainer Grotelüschen
1
, Ryosuke Nakano
2
, Mitsufuji Suguru
3
, Shinichiro Yokota
4
, Philipp Seeger
1
, Hans Christian Schmidt
5
, Jöran Lücke
6
, Dimitra E Zazara
7
, Morsal Sabihi
6
, Eleftherios D Papazoglou
6
, Alessandro Parente
8
, Charles-Henri Wassmer
9
, Nicola Colucci
10
, Panagis M Lykoudis
11
, Ioannis A Ziogas
12
, Dimitrios Moris
13
, Baris Mercanoglu
1
, Stephan Bennemann
14
, Abdulrahman Al-Harazi
15
, Michael F Nentwich
16
, Felix Nickel
1
, Oliver Mann
1
, Jakob R Izbicki
1
, Thilo Hackert
1
, Jun Oh
17
, Maja Segedi
18
, Michael Bleszynski
18
, Stephanie Chartier-Plante
18
, David Harriman
19
, Kim C Wagner
20
, Karl J Oldhafer
20
, Ansgar W Lohse
21
, Petra Arck
22
, Gabriel E Gondolesi
23
, Samuel Huber
24
, Blayne A Sayed
25
, Morgan Vandermeulen
26
, Graziano Oldani
27
, Sasha Belenkov
28
, Jun Li
1
, Christian Tomuschat
29
, Tobias Dust
30
, Ane Miren Andres Moreno
31
, Emmanouil Giorgakis
32
, Megan A Adams
33
, George Mazariegos
34
, Tao Zhang
35
, Angus W Thomson
36
, Anastasios D Giannou
37
Affiliations
- 1 Department of General, Visceral and Thoracic Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 2 Department of Surgery, Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA; Department of Gastroenterological and Transplant Surgery, Graduate School of Biomedical and Health Sciences Hiroshima University, Hiroshima, Japan.
- 3 Department of Surgery, Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA.
- 4 Division of Abdominal Transplant, Department of Surgery, Stanford University School of Medicine, Stanford, CA.
- 5 Research Laboratory W23, Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; SimLab - Students for Surgery, Student research group, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 6 Department of General, Visceral and Thoracic Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Section of Molecular Immunology and Gastroenterology, I. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 7 Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, Hamburg, Germany; University Children's Hospital, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Division for Experimental Feto-Maternal Medicine, Department of Obstetrics and Fetal Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 8 Department of Hepatopancreatobiliary and Liver Transplant Surgery, Queen Elizabeth Hospital, Birmingham, UK; Institute of Liver Studies, King's College Hospital NHS Foundation Trust, London, UK.
- 9 Hepatology and Transplantation Laboratory, Department of Surgery, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
- 10 Department of Surgery, University of Cambridge, Cambridge, UK; Division of General Surgery, Department of Surgery, University of British Columbia, Vancouver, British Columbia, Canada; Hepatopancreatobiliary and Liver Transplant Surgery, Vancouver General Hospital, Vancouver, British Columbia, Canada.
- 11 4th Department of Surgery, National and Kapodistrian University of Athens, Athens, Greece; Division of Surgery & Interventional Science, University College London (UCL), UK.
- 12 Division of Transplant Surgery, Department of Surgery, University of Colorado Anschutz Medical Campus and Children's Hospital Colorado, Aurora, CO.
- 13 MedStar Georgetown Transplant Institute, MedStar Georgetown University Hospital, Washington, DC.
- 14 Semmelweis University, Faculty of Medicine, Department of Surgery, Transplantation, and Gastroenterology, Budapest, Hungary.
- 15 SimLab - Students for Surgery, Student research group, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 16 Department of Intensive Care Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 17 University Children's Hospital, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 18 Division of General Surgery, Department of Surgery, University of British Columbia, Vancouver, British Columbia, Canada; Hepatopancreatobiliary and Liver Transplant Surgery, Vancouver General Hospital, Vancouver, British Columbia, Canada.
- 19 Department of Urologic Sciences, University of British Columbia, Vancouver, Canada.
- 20 Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, Asklepios Hospital Barmbek, Hamburg, Germany; Semmelweis University Budapest, Asklepios Campus Hamburg, Hamburg, Germany.
- 21 Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 22 Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Division for Experimental Feto-Maternal Medicine, Department of Obstetrics and Fetal Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 23 Division of Transplant Surgery, Department of Surgery, University of Colorado Anschutz Medical Campus and Children's Hospital Colorado, Aurora, CO; General Surgery, Liver, Pancreas and Intestinal Transplantation, Hospital Universitario, Fundacion Favaloro, Buenos Aires, Argentina.
- 24 Section of Molecular Immunology and Gastroenterology, I. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 25 Department of Surgery, University of Toronto, Toronto, ON, Canada; HBP and Multi Organ Transplant Program, Division of General Surgery, University Health Network, HPB Surgical Oncology, Toronto, ON, Canada.
- 26 Department of Visceral Surgery and Transplantation, CHU Liege, Liege, Belgium; Centre de Recherche et d'Enseignement du Département de Chirurgie (CREDEC), GIGA Metabolism, University of Liege, Liege, Belgium.
- 27 Hepatology and Transplantation Laboratory, Department of Surgery, Faculty of Medicine, University of Geneva, Geneva, Switzerland; Division of General Surgery, Department of Surgery, University of British Columbia, Vancouver, British Columbia, Canada; Hepatopancreatobiliary and Liver Transplant Surgery, Vancouver General Hospital, Vancouver, British Columbia, Canada.
- 28 PerkinElmer. Inc, Waltham, MA.
- 29 Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 30 Department of Trauma and Orthopedic Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- 31 Transplant Group, La Paz University Hospital Health Research Institute (IdiPAZ), Madrid, Spain; Department of Pediatric Surgery, La Paz University Hospital, Madrid, Spain.
- 32 Division of Abdominal Transplantation, Department of Surgery, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC; Division of Solid Organ Transplant, Department of Surgery, University of Arkansas for Medical Sciences, Little Rock, AR.
- 33 Division of Surgery & Interventional Science, University College London (UCL), UK.
- 34 Department of Transplant Surgery, UPMC Children's Hospital, Pittsburgh, PA; Hillman Center for Pediatric Transplantation, Thomas E. Starzl Transplantation Institute, UPMC Children's Hospital of Pittsburgh, Pittsburgh, PA.
- 35 Department of Gastric Surgery, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, PR China.
- 36 Department of Surgery, Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA; Department of Immunology, University of Pittsburgh, Pittsburgh, PA.
- 37 Department of General, Visceral and Thoracic Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Section of Molecular Immunology and Gastroenterology, I. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Division of General Surgery, Department of Surgery, University of British Columbia, Vancouver, British Columbia, Canada; Hepatopancreatobiliary and Liver Transplant Surgery, Vancouver General Hospital, Vancouver, British Columbia, Canada; Semmelweis University, Faculty of Medicine, Department of Surgery, Transplantation, and Gastroenterology, Budapest, Hungary; Department of Intensive Care Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Semmelweis University Budapest, Asklepios Campus Hamburg, Hamburg, Germany; Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. Electronic address: a.giannou@uke.de.
- PMID: 41354508
- DOI: 10.1016/j.cpsurg.2025.101920
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Declaration of competing interest The authors of this manuscript have no conflicts of interest to disclose.
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