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Comparative Study
. 1974 Jul;28(1):156-7.
doi: 10.1128/am.28.1.156-157.1974.

Improved techniques for staining and enumerating focus formation in viral infectivity assays

D J Self, K F Pettiss, D D Bigner
Comparative Study

Improved techniques for staining and enumerating focus formation in viral infectivity assays

D J Self et al. Appl Microbiol. 1974 Jul.

Abstract

Improved techniques consisting of glutaraldehyde fixation, Giemsa staining, and colony counter enumeration of foci in viral infectivity assays were compared to microscope scanning and lantern slide projection methods. These techniques broadened the scope of application to potentially hazardous viruses and increased the efficiency of assaying.

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References

    1. Appl Microbiol. 1973 Jan;25(1):149-50 - PubMed
    1. Virology. 1956 Dec;2(6):838-40 - PubMed
    1. Virology. 1958 Dec;6(3):669-88 - PubMed

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