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. 1977 Dec 23;39(3):315-9.
doi: 10.1007/BF00295426.

Characterization of residual hexosaminidase activity in Sandhoff's disease using man-Chinese hamster cell hybrids

H L Hoeksema, A J Reuser, A T Hoogeveen, A Westerveld, H Galjaard

Characterization of residual hexosaminidase activity in Sandhoff's disease using man-Chinese hamster cell hybrids

H L Hoeksema et al. Hum Genet. .

Abstract

To obtain information about the nature of the residual hexosaminidase activity in Sandhoff's disease, hybrid cell lines between fibroblasts from a patient with Sandhoff's disease and Chinese hamster cells were isolated. In these hybrid cell lines, a heteropolymeric isoenzyme was detected that is composed of human alpha- and Chinese hamster hexosaminidase subunits. Due to the electrophoretic and immunological behavior of the heteropolymeric molecules in interspecies hybrids with normal fibroblasts and with cells from a patient with Sandhoff's disease, we conclude that Sandhoff cells contain an alpha-subunit of hexosaminidase with normal characteristics.

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References

    1. Biochem J. 1968 Apr;107(3):321-7 - PubMed
    1. Nat New Biol. 1971 Nov 3;234(44):20-4 - PubMed
    1. Ann Hum Genet. 1975 Jul;39(1):111-23 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1569-73 - PubMed
    1. Nature. 1974 Aug 16;250(467):580-2 - PubMed