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. 1974 Oct;71(10):3829-33.
doi: 10.1073/pnas.71.10.3829.

The length of the terminal repetition in adenovirus-2 DNA

R J Roberts, J R Arrand, W Keller

The length of the terminal repetition in adenovirus-2 DNA

R J Roberts et al. Proc Natl Acad Sci U S A. 1974 Oct.

Abstract

Adenovirus-2 DNA was end-labeled by partial digestion with Escherichia coli exonuclease III and resynthesis with the DNA polymerase from avian myeloblastosis virus and alpha-(32)P-labeled deoxyribonucleoside triphosphates. This end-labeled DNA was cleaved with several specific endonucleases and the terminal fragments were characterized by gel electrophoresis and pyrimidine tract analysis. Two endonucleases gave identical fragments from both ends, presumably from cleavage within the inverted terminal repetition, while all other endonucleases gave dissimilar fragments from the two ends. From the sizes of these fragments it is estimated that the inverted terminal repetition is between 100 and 140 nucleotide pairs long.

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References

    1. Nucleic Acids Res. 1974 Jun;1(6):727-52 - PubMed
    1. Proc Natl Acad Sci U S A. 1967 May;57(5):1302-9 - PubMed
    1. J Biol Chem. 1969 Jun 10;244(11):2996-3008 - PubMed
    1. Eur J Biochem. 1969 Dec;11(2):395-9 - PubMed
    1. J Mol Biol. 1970 Jul 28;51(2):379-91 - PubMed

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