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. 1974 Oct;71(10):3892-5.
doi: 10.1073/pnas.71.10.3892.

Enzymatic synthesis of solid phase-bound DNA sequences corresponding to specific mammalian genes

P Venetianer, P Leder

Enzymatic synthesis of solid phase-bound DNA sequences corresponding to specific mammalian genes

P Venetianer et al. Proc Natl Acad Sci U S A. 1974 Oct.

Abstract

With oligo(dT)-cellulose as primer, RNA-dependent DNA polymerase catalyzes the synthesis of cellulose-bound DNA that is complementary to mouse globin mRNA. The resulting cellulose-bound or solid phase complementary DNA hybridizes specifically with globin mRNA and permits the recovery of intact globin mRNA. This simple technique for the synthesis of solid phase-bound complementary DNA provides an additional and convenient method for the purification of specific genetic sequences.

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References

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