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. 1974 Oct;71(10):3998-4001.
doi: 10.1073/pnas.71.10.3998.

Heterogeneity of HL-A antigen preparations is due to variable sialic acid content

P Parham, R E Humphreys, M J Turner, J L Strominger

Heterogeneity of HL-A antigen preparations is due to variable sialic acid content

P Parham et al. Proc Natl Acad Sci U S A. 1974 Oct.

Abstract

Purified, papain-solubilized preparations of HL-A antigen from cultured human lymphoblastoid cells (HL-A2 and HL-A7,12 from RPMI 4265 cells and a mixture of HL-A3, W-25, HL-A12, and HL-A27 from IM-1 cells) show substantial charge heterogeneity in isoelectric focusing gels. This heterogeneity can be ascribed largely to variable numbers of sialic acid residues on each molecule. Neuraminidase (EC 3.2.1.18) treatment of the HL-A antigens as a function of time altered the band patterns in a manner demonstrating that up to three sialic acid residues are present on both first locus ("LA") and second locus ("Four") antigens. Neuraminidase treatment did not alter the specificity or specific activity of the purified antigens.

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References

    1. Nature. 1968 Jul 13;219(5150):184-6 - PubMed
    1. J Chromatogr. 1968 Aug 27;36(3):362-5 - PubMed
    1. Science. 1971 Aug 6;173(3996):542-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1394-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 May;70(5):1603-7 - PubMed