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Comparative Study
. 1974 Dec;28(6):1040-6.
doi: 10.1128/am.28.6.1040-1046.1974.

In vitro system for production of mouse mammary tumor virus

D L Fine, L O Arthur, J K PLOWMAN, E A Hillman, F Klein
Comparative Study

In vitro system for production of mouse mammary tumor virus

D L Fine et al. Appl Microbiol. 1974 Dec.

Abstract

An in vitro system for production, purification, and concentration of mouse mammary tumor virus is described. Monolayer cultures of C(3)H mouse mammary tumor cells propagated at 34 C in roller bottles in the presence of dexamethasone, a glucocorticoid hormone, release B-type particles which possess ribonucleic acid and a ribonucleic acid-dependent deoxyribonucleic acid polymerase. One thousandfold concentration by ultracentrifugation with subsequent gradient fractionation yielded > 7 x 10(10) particles per ml in the 1.16- to 1.18-g/ml region. Mouse mammary tumor virus produced in this system was free of detectable C-type virus.

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References

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