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. 1977 Dec;4(12):4291-303.
doi: 10.1093/nar/4.12.4291.

Nucleotide sequence of a lysine tRNA from Bacillus subtilis

Y Yamada, H Ishikura
Free PMC article

Nucleotide sequence of a lysine tRNA from Bacillus subtilis

Y Yamada et al. Nucleic Acids Res. 1977 Dec.
Free PMC article

Abstract

A lysine tRNA (tRNA1Lys) was purified from Bacillus subtilis W168 by a consecutive use of several column chromatographic systems. The nucleotide sequence was determined to be pG-A-G-C-C-A-U-U-A-G-C-U-C-A-G-U-D-G-G-D-A-G-A-G-C-A-U-C-U-G-A-C-U-U(U*)-U-U-K-A-psi-C-A-G-A-G-G-m7G(G)-U-C-G-A-A-G-G-T-psi-C-G-A-G-U-C-C-U-U-C-A-U-G-G-C-U-C-A-C-C-AOH, where K and U* are unidentified nucleosides. The nucleosides of U34 and m7G46 were partially substituted with U* and G, respectively. The binding ability of lysyl-tRNA1Lys to Escherichia coli ribosomes was stimulated with ApApA as well as ApApG.

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References

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