Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Dec;74(12):5350-4.
doi: 10.1073/pnas.74.12.5350.

Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes

J C Alwine, D J Kemp, G R Stark

Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes

J C Alwine et al. Proc Natl Acad Sci U S A. 1977 Dec.

Abstract

We describe a technique for transferring electrophoretically separated bands of RNA from an agarose gel to paper strips. The RNA is coupled covalently to diazobenzyloxymethyl groups on the paper. After transfer and appropriate treatment of the paper to destroy remaining diazo groups, specific RNA bands can be detected by hybridization with 32P-labeled DNA probes followed by autoradiography. This procedure allows detection of specific RNA bands with high sensitivity and low background.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1951 Sep;37(9):575-8 - PubMed
    1. Biokhimiia. 1961 Sep-Oct;26:934-42 - PubMed
    1. Biokhimiia. 1959 Jan-Feb;24(1):144-56 - PubMed
    1. J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
    1. Cell. 1976 Jun;8(2):163-82 - PubMed

Publication types

LinkOut - more resources