Immunoelectrophoretic analysis and radial immunodiffusion assay using plasminogen purified from fresh human plasma
- PMID: 4143118
- PMCID: PMC1554026
Immunoelectrophoretic analysis and radial immunodiffusion assay using plasminogen purified from fresh human plasma
Abstract
Plasminogen was purified from fresh human plasma by affinity chromatography and gel filtration and was characterized functionally, electrophoretically and on a weight basis. After antibody raised against this material was demonstrated to be monospecific for plasminogen/plasmin, it was employed in an immunoelectrophoretic analysis of plasminogen activated in several ways and in a radial immunodiffusion assay of human plasma plasminogen, where the mean level found was 476 μg/ml.
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