Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Dec 26;20(12):e0339345.
doi: 10.1371/journal.pone.0339345. eCollection 2025.

Genetic association of microRNA-146a polymorphisms with the severity of coronary artery lesions in acute myocardial infarction

Toan Hoang Ngo  1 , Chuong Quoc Ho  2 , Son Kim Tran  1
Affiliations

Genetic association of microRNA-146a polymorphisms with the severity of coronary artery lesions in acute myocardial infarction

Toan Hoang Ngo et al. PLoS One. .

Abstract

Background: Polymorphisms situated within the microRNA-146a gene have been extensively reported to fulfill a crucial regulatory function in controlling inflammatory responses and modulating gene expression. More recently, these specific genetic variants have also been implicated in the pathogenesis of coronary artery lesions; nevertheless, the current body of available evidence remains notably sparse.

Objectives: To investigate the characteristics of the microRNA-146a gene polymorphisms rs2431697, rs57095329, and rs2910164, and their association with the severity of coronary artery lesions.

Materials and methods: This comparative cross-sectional study included patients with acute myocardial infarction (AMI) and a control group, recruited from two hospitals in Can Tho City, Vietnam, between October 2023 and May 2025. All participants underwent clinical evaluation and coronary angiography. Subsequently, the microRNA-146a gene polymorphisms-rs2431697, rs57095329, and rs2910164-were analyzed using gene sequencing.

Results: A total of 249 patients were included in the AMI group and 249 in the control group. The mean age of the patient cohort (66.65 ± 10.89 years) was similar to that of the controls (66.67 ± 14.12 years). Analysis of the rs2910164 polymorphism showed a lower frequency of the GG genotype in the AMI group compared with the control group (14.9% vs. 20.9%, p < 0.05), and all three SNPs met Hardy-Weinberg equilibrium criteria. Among patients with AMI, 30.5% had three-vessel coronary artery disease, and severe stenosis (≥90% luminal narrowing) was present in 78.3% of cases. Multivariate analysis demonstrated that the rs2431697 CC + TC genotype (OR = 0.10), the rs2910164 GG + CG genotype (OR = 3.23), diabetes mellitus (OR = 5.14), dyslipidemia (OR = 4.01), smoking (OR = 5.16), NT-proBNP ≥ 300 pg/mL (OR = 8.69), a GRACE score > 140 (OR = 10.82), and a TIMI score > 4 (OR = 6.50) were independent predictors of severe coronary stenosis (p < 0.05).

Conclusion: Patients with acute myocardial infarction had a lower prevalence of the GG genotype of the rs2910164 polymorphism compared with the control group. The rs2910164 polymorphism of microRNA-146a-particularly genotypes carrying the G allele-along with a history of diabetes mellitus, dyslipidemia, smoking, NT-proBNP ≥ 300 pg/mL, a GRACE score > 140, and a TIMI score > 4 were identified as independent predictors of severe coronary artery stenosis. In contrast, the rs2431697 polymorphism with genotypes carrying the C allele was found to be a protective factor.

PubMed Disclaimer

Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Comparison of GRACE and TIMI scores according to the severity of coronary artery stenosis and the presence of triple-vessel disease.
See this image and copyright information in PMC

References

    1. Khan MA, Hashim MJ, Mustafa H, Baniyas MY, Al Suwaidi SKBM, AlKatheeri R, et al. Global epidemiology of ischemic heart disease: results from the global burden of disease study. Cureus. 2020;12(7):e9349. doi: 10.7759/cureus.9349 - DOI - PMC - PubMed
    1. Salari N, Morddarvanjoghi F, Abdolmaleki A, Rasoulpoor S, Khaleghi AA, Hezarkhani LA, et al. The global prevalence of myocardial infarction: a systematic review and meta-analysis. BMC Cardiovasc Disord. 2023;23(1):206. doi: 10.1186/s12872-023-03231-w - DOI - PMC - PubMed
    1. Singh B, Singh A, Goyal A, Chhabra S, Tandon R, Aslam N, et al. The prevalence, clinical spectrum and the long term outcome of ST-segment elevation myocardial infarction in young - a prospective observational study. Cardiovasc Revasc Med. 2019;20(5):387–91. doi: 10.1016/j.carrev.2018.07.020 - DOI - PubMed
    1. Hoang Ngo T, Tran Khuong Nguyen N, Thi Ngoc Pham N, Tran BLT, Tuan Huynh A, Duy Nguyen K, et al. The combination of CYP2C19 polymorphism and inflammatory cell ratios in prognosis cardiac adverse events after acute coronary syndrome. Int J Cardiol Cardiovasc Risk Prev. 2023;19:200222. doi: 10.1016/j.ijcrp.2023.200222 - DOI - PMC - PubMed
    1. Leitão AL, Enguita FJ. A Structural view of miRNA biogenesis and function. Noncoding RNA. 2022;8(1):10. doi: 10.3390/ncrna8010010 - DOI - PMC - PubMed