Maximizing the Value of Preclinical and Translational Animal Research Models via Next-Generation Standardized Reference Diets: a White Paper from the Laboratory Animal Nutrition Taskforce for an Education and Research Network

Abstract

A new generation of standard reference diets (SRDs) is essential to enhance the integrity of research associated with the use of laboratory animal models in the study of human disease. Support for the value of this fundamental facet of experimental methodology is provided via a historical overview, an assessment of current status, and the advancements and opportunities that will be realized through further development and routine use. An outline of the extensive investment in animal nutrition research by National Institutes of Health during the past 30 y is presented with specific reference to rodent animal models and standardization in dietary methodology. Timely recommendations for renewed action that revitalize and expand the use of SRDs in rodents and other models, such as zebrafish, are provided with the aim of achieving more rigor, transparency, replicability, interpretability, and integrity in preclinical and translational animal model-based research. Ultimately, the benefits of these efforts will be fulfilled through expeditious development of prophylactic and therapeutic approaches for human diseases.

Keywords: diets; feed; mouse; natural ingredient; purified ingredient; rat; replicability; reproducibility; rodent; standardized reference diet; zebrafish.