Correlation Study of LncRNA H19 Single Nucleotide Polymorphism With Delayed Graft Function of Transplanted Kidney: A Retrospective Study

Abstract

Objective: To investigate the association between LncRNA H19 single nucleotide polymorphisms and delayed graft function.

Methods: This retrospective study included 827 kidney transplantations performed between January 1, 2015, and December 31, 2022, and fifty recipients with delayed graft function were assigned to the DGF group, while fifty recipients with immediate graft function were assigned to the IGF group. The DGF recipients and the IGF recipients had donor kidneys from the same donor. Single nucleotide polymorphisms (SNPs) of LncRNA H19 (rs217727, rs2067051, rs2251375, rs492994, rs2839698, and rs10732516) were genotyped using peripheral blood samples, rs2067051, rs2251375, rs492994, rs2839698 and rs10732516.

Results: Patients in the DGF group had 43 homozygous, 2 wild-type, and 5 heterozygous rs492994 gene loci, and 38 homozygous, 2 wild-type, and 10 heterozygous rs2839698 gene loci. Patients in the IGF group had 34 homozygous, 2 wild-type, and 14 heterozygous rs492994 gene loci, and 48 homozygous, 1 wild-type, and 1 heterozygous rs2839698 gene loci. There were differences in SNP between the DGF group and the IGF group at the rs492994 and rs2839698 gene loci, with P < .05 and statistically significant differences. However, there was no statistically significant difference in SNP at the other 4 gene loci, with P > .05. Since the donor kidneys in the DGF group and the IGF group come from the same donor, their donor data are identical and there are no differences. However, there were no significant differences between the 2 groups in donor kidney laterality, cold ischemia time, vascular anastomosis time, or key recipient characteristics (e.g., age, gender, body mass index, HLA mismatch rate) (all P > .05).

Conclusion: The polymorphisms rs492994 and rs2839698 in LncRNA H19 are significantly associated with the occurrence of DGF after kidney transplantation.